| 质粒名称: | pJL 89 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 98558 |
| 存储实验室: | John Lindbo |
|
载体骨架基本信息 |
|
| 载体名称: | pCB301 |
| 载体出品公司: | -- |
| 空载体大小: | 4500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 在35S启动子转录起始位点产生StuI位点，在35S终止子上游插入来自三角洲肝炎病毒的SmaI位点和核酶。 |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | Enh 35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJL 89 was a gift from John Lindbo (Addgene plasmid # 98558 ; http://n2t.net/addgene:98558 ; RRID:Addgene_98558) |
| 参考文献部分: | TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Lindbo JA. Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24. 10.1104/pp.107.106377 PubMed 17720752 |
买家导航
pJL89载体基本信息
pJL89载体图谱质粒图谱和多克隆位点信息
pJL89载体简介
pJL89载体序列
LOCUS pJL 89 4707 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Useful for making infectious clones of RNA virus cDNAs; Has 35S
promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium
vector..
ACCESSION .
VERSION .
KEYWORDS pJL 89
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4707)
AUTHORS Lindbo JA
TITLE TRBO: a high-efficiency tobacco mosaic virus RNA-based
overexpression vector.
JOURNAL Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
PUBMED 17720752
REFERENCE 2 (bases 1 to 4707)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4707
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(156..1304)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(1603..2397)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 2606..3137
/label=oriV
/note="incP origin of replication"
rep_origin 2614..3228
/label=oriV
/note="origin of replication for the bacterial F plasmid"
misc_feature 3321..3345
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
primer_bind 3425..3441
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 3426..3442
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4161..4181
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
promoter 4170..4215
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 4685..2
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 acctaagaga aaagagcgtt tattagaata atcggatatt taaagggcgt gaaaaggttt
61 atccgttcgt ccatttgtat gtgcatgcca accacaggag atctcagtaa agcgctggct
121 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
181 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
241 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
301 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
361 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
421 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
481 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
541 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
601 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
661 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
721 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
781 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
841 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
901 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
961 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
1021 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
1081 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
1141 tcgatcttgg ccgtagcttg ctgggccatc gagccgacgg actggaaggt ttcgcggggc
1201 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
1261 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
1321 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
1381 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
1441 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
1501 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
1561 cgctcctgct tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag
1621 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
1681 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
1741 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
1801 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
1861 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatgggg tgtcttcttc
1921 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
1981 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
2041 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
2101 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
2161 aaggtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
2221 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
2281 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
2341 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
2401 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
2461 acgaactcca attcactgtt ccttgcattc taaaacttta aataccagaa aacagctttt
2521 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca
2581 caattatggg tgatgctgca actcgagcgg gccgggaggg ttcgagaagg gggggcaccc
2641 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
2701 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
2761 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
2821 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
2881 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
2941 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
3001 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
3061 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
3121 ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc
3181 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt
3241 ctctagtgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
3301 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
3361 caacttaata acacattgcg gacgttttta aagtactggg gtggttttgg taccgggccc
3421 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcaggt caacatggtg
3481 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
3541 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
3601 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
3661 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
3721 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
3781 caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc caaaaatatc
3841 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
3901 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
3961 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
4021 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
4081 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
4141 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
4201 tttcatttgg agaggcctcc ttttttccta ggatgcagcc cgggtcggca tggcatctcc
4261 acctcctcgc ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag
4321 ggagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
4381 tcctgttgcc ggtcttgcga ctagagtccg caaaaatcac cagtctctct ctacaaatct
4441 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
4501 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
4561 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
4621 agcccggccg ggggatccac tagcagattg tcgtttcccg ccttcagttt aaactatcag
4681 tgtttgacag gatatattgg cgggtaa
//
